| AMY1

Amylase Alpha 1, Salivary (AMY1) is an enzyme present in many organisms that is an isoenzyme of a amylase produced by the salivary glands. Amylases are enzymes that hydrolyze or breakdown starch into oligosaccharides such as dextrin and maltose. a-Amylase, a major protein in saliva, catalyzes the hydrolysis of 1, 4-á-glucosidic linkages in starch and other polysaccharides leading to the formation of smaller oligosaccharides, dextrins and maltoses. The enzyme also plays an important role in the colonization and metabolism of bacteria leading to the formation of dental plaque.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	400	200	100	50	25	12.5	6.25	0
OD	2.15	1.63	1.13	0.71	0.5	0.33	0.23	0.07
Corrected OD	2.08	1.56	1.06	0.64	0.43	0.26	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human AMY1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Saliva(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human AMY1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Saliva(n=4)
1 : 2	Range (%)	84-95	88-96	90-111
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108